Non-Ideality by Sedimentation Velocity of Halophilic Malate Dehydrogenase in Complex Solvents
نویسندگان
چکیده
منابع مشابه
Crystallization of halophilic malate dehydrogenase from Halobacterium marismortui.
Malate dehydrogenase from the extreme halophile Halobacterium marismortui crystallizes in highly concentrated phosphate solution in space group 12 with cell dimensions a = 113.8 A, b = 122.8 A, c = 126.7 A, beta = 98.1 degrees. The halophilic enzyme was found to be unstable at lower concentrations of phosphate. It associates with unusually large amounts of water and salt, and the combined parti...
متن کاملStructural features that stabilize halophilic malate dehydrogenase from an archaebacterium.
The high-resolution structure of halophilic malate dehydrogenase (hMDH) from the archaebacterium Haloarcula marismortui was determined by x-ray crystallography. Comparison of the three-dimensional structures of hMDH and its nonhalophilic congeners reveals structural features that may promote the stability of hMDH at high salt concentrations. These features include an excess of acidic over basic...
متن کاملTHE EFFECT OF SALT STRESS ON MALATE DEHYDROGENASE IN WHEAT
Effect of various NaCI treatments (0, 50, 100, 200 and 300 mM) at different growth and development stages (tillering, boot swollen, flowering and anthesis) of two wheat cultivars on the kinetic activity and PAGE electrophoretic pattern of leaf malate dehydrogenase was studied under greenhouse conditions. Ghods was salt-sensitive and Boolani was salt-tolerant. In general, in response to salinti...
متن کاملOn the non ideality of CO2 solutions in ionic liquids and other low volatile solvents
The nonideality of CO2 solutions in ionic liquids and other low volatile solvents, with which CO2 is known to form electron donor-acceptor (EDA) complexes, is here investigated. It is shown that the deviations from the ideality observed are not related with the stability of the EDA complex formed, and inmost cases these deviations are small and dominated by entropic effects. For this reason, wh...
متن کاملMalate dehydrogenase isolated from extremely halophilic bacteria of the Dead Sea. 1. Purification and molecular characterization.
The complete purification of malate dehydrogenase (EC 1.1.1.37) from extremely halophilic bacteria of the Dead Sea is described. The purification procedure includes (a) precipitation by ammonium sulfate, (b) fractionation on Sepharose 4B using a decreasing concentration gradient of ammonium sulfate, (c) gel permeation chromatography on Sephadex G-100, (d) chromatography on hydroxylapatite, and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2001
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(01)75838-9